Just some of the protocols I used in the CrabLab.
Imaging
- Di-4 ANEPPS: 20 µl to 1ml in well around desheathed STG for 20 minutes
- Use 20 x objective
- One high res picture + 3 low res pictures
Dopamine experiment
- Start with 10-6M dopamine solution.
- 10 minutes perfusion
- Image: 1 high res + 3 low res
- 10-4 DA solution
- 10 minutes perfusion
- Image: 1 high res + 3 low res
- Washout 15 minutes with normal saline
- Image: 1 high res + 3 low res
Pilo
- Cut stn
- Image
- Apply Pilocarpine
- Perfuse 10 minutes
- Image